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【流式學(xué)習(xí)】γδT細(xì)胞：雖然我很少，但我很重要
Human：通常，人的γδT細(xì)胞根據(jù)其TCR基因重排過程中TCR δ鏈的不同主要分為兩大亞群：Vδ1T細(xì)胞亞群和Vδ2T細(xì)胞亞群，其中，與Vδ1T細(xì)胞對(duì)應(yīng)的TCRγ鏈主要為VγI基因家族成員（Vγ2/3/4/5/8），主要分布于黏膜和皮下組織中，在不同的組織中數(shù)量不等；Vδ2 T 細(xì)胞是γδ T 細(xì)胞的主要細(xì)胞群，其TCRγ鏈主要為VγII基因家族成員(Vγ9)，因此又叫 Vγ9Vδ2T 細(xì)胞，僅存在于靈長(zhǎng)類動(dòng)物和人體內(nèi)，主要分布于外周血中，占外周血淋巴細(xì)胞的0.5% ～10%。
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先通過FSC-A/SSC-A圈中淋巴細(xì)胞；
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通過FSC-A/W排除粘連體；
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通過SSC-A/W排除粘連體；
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通過EMA死活染料排除掉死細(xì)胞；
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圈中CD3＋T淋巴細(xì)胞；
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在CD3＋T淋巴細(xì)胞中接著分析CD4＋T細(xì)胞和CD8＋T細(xì)胞的比例；因?yàn)槿送庵苎?/span>γδT細(xì)胞的表型基本都是CD4－CD8－，所以圈出CD4－CD8－雙陰性T細(xì)胞，準(zhǔn)備分析γδT表型；
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圈中泛γδT細(xì)胞；
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分析泛γδT細(xì)胞中的Vδ1＋T細(xì)胞和Vδ2＋T細(xì)胞 2個(gè)亞群的比例，以及Vδ1－Vδ2－T雙陰性細(xì)胞的比例；
|
Item |
Cat |
Size |
Clone |
|
APC anti-human TCR γ/δ |
331212 |
100 tests |
B1 |
|
FITC anti-human TCR γ/δ |
331208 |
100 tests |
B1 |
|
PE anti-human TCR γ/δ |
331210 |
100 tests |
B1 |
|
PE/Cy7 anti-human TCR γ/δ |
331222 |
100 tests |
B1 |
|
PerCP/Cyanine5.5 anti-human TCR γ/δ |
331224 |
100 tests |
B1 |
|
Purified anti-human TCR γ/δ |
331202 |
100 ug |
B1 |
|
LEAF? Purified anti-human TCR γ/δ |
331204 |
500ug |
B1 |
|
Biotin anti-human TCR γ/δ |
331206 |
100ug |
B1 |
|
Alexa Fluor? 647 anti-human TCR γ/δ |
331214 |
100 tests |
B1 |
|
Brilliant Violet 421? anti-human TCR γ/δ |
331218 |
100 tests |
B1 |
|
Brilliant Violet 510? anti-human TCR γ/δ |
331220 |
100 tests |
B1 |
|
PE/Dazzle? 594 anti-human TCR γ/δ |
331226 |
100 tests |
B1 |
|
APC/Fire? 750 anti-human TCR γ/δ |
331228 |
100 tests |
B1 |
|
Purified anti-human TCR Vγ9 |
331301 |
25ug |
B3 |
|
FITC anti-human TCR Vγ9 |
331306 |
100 tests |
B3 |
|
PE anti-human TCR Vγ9 |
331308 |
100 tests |
B3 |
|
APC anti-human TCR Vγ9 |
331310 |
100 tests |
B3 |
|
PerCP anti-human TCR Vδ2 |
331410 |
100 tests |
B6 |
|
FITC anti-human TCR Vδ2 |
331406 |
100 tests |
B6 |
|
Pacific Blue? anti-human TCR Vδ2 |
331414 |
100 tests |
B6 |
|
Alexa Fluor? 700 anti-human TCR Vδ2 |
331416 |
100 tests |
B6 |
|
APC anti-human TCR Vδ2 |
331418 |
100 tests |
B6 |
|
APC/Fire? 750 anti-human TCR Vδ2 |
331420 |
100 tests |
B6 |
|
PE/Cy7 anti-human TCR Vδ2 |
331422 |
100 tests |
B6 |
|
PerCP/Cyanine5.5 anti-human TCR Vδ2 |
331424 |
100 tests |
B6 |
|
Brilliant Violet 421? anti-human TCR Vδ2 |
331428 |
100 tests |
B6 |
|
Brilliant Violet 605? anti-human TCR Vδ2 |
331430 |
100 tests |
B6 |
|
Brilliant Violet 510? anti-human TCR Vδ2 |
331432 |
100 tests |
B6 |
|
PE/Dazzle? 594 anti-human TCR Vδ2 |
331426 |
100 tests |
B6 |
|
Brilliant Violet 711? anti-human TCR Vδ2 |
331412 |
100 tests |
B6 |
|
PE anti-human TCR Vδ2 |
331408 |
100 tests |
B6 |
|
Purified anti-human TCR Vδ2 |
331402 |
100 ug |
B6 |
|
Biotin anti-human TCR Vδ2 |
331404 |
100 ug |
B6 |
|
Purified anti-mouse TCR γ/δ |
107502 |
500ug |
UC7-13D5 |
|
Brilliant Violet 421? anti-mouse TCR γ/δ |
118119 |
125ul |
GL3 |
|
FITC anti-mouse TCR γ/δ |
118106 |
200 ug |
GL3 |
|
Biotin anti-mouse TCR γ/δ |
118103 |
50 ug |
GL3 |
|
LEAF? Purified anti-mouse TCR γ/δ |
107506 |
500 ug |
UC7-13D5 |
|
Alexa Fluor? 488 anti-mouse TCR γ/δ |
107512 |
100 ug |
UC7-13D5 |
|
APC anti-mouse TCR γ/δ |
118116 |
100 ug |
GL3 |
|
PE anti-mouse TCR γ/δ |
118108 |
100 ug |
GL3 |
|
FITC anti-mouse TCR γ/δ |
107504 |
500 ug |
UC7-13D5 |
|
PE anti-mouse TCR γ/δ |
107508 |
100 ug |
UC7-13D5 |
|
Purified anti-mouse TCR γ/δ |
118101 |
100 ug |
GL3 |
|
LEAF? Purified anti-mouse TCR γ/δ |
118114 |
500 ug |
GL3 |
|
PerCP/Cyanine5.5 anti-mouse TCR γ/δ |
118118 |
100 ug |
GL3 |
|
Brilliant Violet 421? anti-mouse TCR γ/δ |
118120 |
50 ug |
GL3 |
|
PE/Cy7 anti-mouse TCR γ/δ |
118124 |
100 ug |
GL3 |
|
Alexa Fluor? 488 anti-mouse TCR γ/δ |
118128 |
100 ug |
GL3 |
|
Brilliant Violet 605? anti-mouse TCR γ/δ |
118129 |
50 ug |
GL3 |
|
Brilliant Violet 510? anti-mouse TCR γ/δ |
118131 |
50 ug |
GL3 |
|
Alexa Fluor? 647 anti-mouse TCR γ/δ |
118134 |
100 ug |
GL3 |
|
APC/Fire? 750 anti-mouse TCR γ/δ |
118136 |
100 ug |
GL3 |
|
APC anti-mouse TCR Vγ1.1/Cr4 Antibody |
141108 |
100 ug |
2.11 |
|
FITC anti-mouse TCR Vγ1.1/Cr4 Antibody |
141104 |
100 ug |
2.11 |
|
PE anti-mouse TCR Vγ1.1/Cr4 Antibody |
141106 |
100 ug |
2.11 |
|
PerCP/Cyanine5.5 anti-mouse TCR Vγ1.1/Cr4 Antibody |
141112 |
100 ug |
2.11 |
|
Pacific Blue? anti-mouse TCR Vγ1.1/Cr4 Antibody |
141110 |
100 ug |
2.11 |
|
Purified anti-mouse TCR Vγ1.1/Cr4 Antibody |
141102 |
100 ug |
2.11 |
|
APC anti-mouse TCR Vγ2 Antibody |
137708 |
100 ug |
UC3-10A6 |
|
FITC anti-mouse TCR Vγ2 Antibody |
137704 |
200 ug |
UC3-10A6 |
|
PE anti-mouse TCR Vγ2 Antibody |
137706 |
200 ug |
UC3-10A6 |
|
APC anti-mouse TCR Vγ3 Antibody |
137506 |
100 ug |
536 |
|
PE anti-mouse TCR Vγ3 Antibody |
137504 |
100 ug |
536 |
|
APC anti-mouse TCR Vδ6.3 Antibody |
154806 |
100 ug |
C504.17C |
|
FITC anti-mouse TCR Vδ6.3 Antibody |
154808 |
100 ug |
C504.17C |
|
PE anti-mouse TCR Vδ6.3 Antibody |
154804 |
100 ug |
C504.17C |
|
Purified anti-mouse TCR Vδ6.3 Antibody |
154802 |
500 μg |
C504.17C |
|
PE anti-mouse TCR Vδ4 Antibody |
134905 |
25 μg |
GL2 |
|
PE anti-mouse TCR Vγ1.1 + Vγ1.2 Antibody |
142704 |
100 ug |
4B2.9 |
|
Purified anti-mouse TCR Vγ1.1 + Vγ1.2 Antibody |
142702 |
100 ug |
4B2.9 |
|
PE anti-rat TCR γ/δ |
202605 |
50 ug |
V65 |
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Gamma-delta(γδ) T cells: friend or foe in cancer development? J Transl Med. -
Six-of-the-best:unique contributions of γδ T cells toimmunology. Nat Rev Immunol. -
OMIP‐020:Phenotypic characterization of human γδ T‐cells by multicolor flow cytometry. Cytometry A.
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